Flow Cytometry 2013 Module 3 – Preprocessing and Quality Assurance of FCM Data
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Preprocessing
- Removing margin events
- Data transformation: log vs. biexponential
- Data normalization
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Quality Assurance
- Overview of quality assurance concepts: total raw/viable cell count, margin event count, outlier detection based on density of common parameters
- Building quality assurance objects using flowQ and generating summary HTML reports