Flow Cytometry 2013 Module 5 – 1D Dynamic gating
- Using quantiles to set a gate based on a negative control
- Using median and standard deviation to set a gate based on a negative control
- Exploring rangeGate: an automated way to set a 1D gate for each sample individually
- Recording proportions of cells in subpopulations of interest by creating a spreadsheet fi le
- Recording a visual record of the gating results by creating JPEG image fi les